AbClon

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Antigen services

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Protein expression purification service

AbClon produce recombinant proteins and antibodies that are important to your research for
convenient use. Protein expression purification service can be performed in conjunction with
gene synthesis and cloning services using various vectors. On the small scale, the optimal
expression and purification conditions are confirmed, and mass production and purification
are carried out to produce efficient protein. Depending on the purpose of the study, it can be
carried out in bacteria and mammalian systems.

Mammalian expression: HEK293F, CHO(option)

Tag Tablet : Fc or His Tag

Bacterial expression: BL21(DE3), BL21(DE3)pLysS, BL21(DE3) RIPL

Tag Tablet : His or GST tag

Service Flow

Mammalian expression

  • 30ml x 2 Condition Transfection & Culture
    • · Expression pattern test
    • · 2~3 weeks
  • 30m x 2 Culture sup. Purification
    • · Purification efficiency test
    • · Fc or His Tag
    • · 1~2 weeks
  • 1L transfection &
    culture & Puri.
    • · scale up(1~10L)
    • · Fc Tag or His Tag
    • · 5~7 weeks
  • Quality control
    • · SDS-PAGE
      confirmation
    • · One week
  • Delivery or
    Next process
    • · report

Bacterial expression

  • Optimization for
    expression
    • · Expression pattern
      and purification
      test
    • · 1~2 weeks
  • 2L culture
    • · Scale up(2~50L)
    • · 1~2 weeks
  • Purification
    • · His or GST Tag
    • · 1~2 weeks
  • Quality control
    • · SDS-PAGE
      confirmation
    • · One week
  • Delivery or
    Next process
    • · report

Features

  • · Production of proteins and antibodies similar to native proteins: Mammalian expression
  • · Rapid protein production at low cost: Bacterial expression

Customer Preparation

  • Cloned vector
  • Vector information
  • Cloning information
  • Signal peptide & Kozak sequence information (Mammalian expression)
  • Tag information
  • Isotype information (antibody production)

Peptide synthesis services

High quality peptides can be synthesized in the form desired by customers with various purity
(~ 98%), quantity (mg ~ kg) and length (~ 150mer), and modified peptide synthesis is also
possible. We also provide free peptide sequence analysis and prediction services based on the
know-how of various antibody production methods for the purpose of producing antigens.

Service range

  • Peptide Sequencing and Prediction
  • Simple synthesis to complex deformation synthesis
  • Linear to circular synthesis
  • Mass synthesis
  • Various purity (> 70%,> 75%,> 80%,> 85%,> 90%,> 95%,> 98%
  • Various modifications (Biotinylation, Phosphorylation, Acetylation, Methylation, Dye labeling, etc.)
  • Peptide library production

Service Flow

  • Synthesis
    • · Solid-Phase Peptide Synthesis
  • Purification & Quality Control
    • · MS
    • · HPLC
  • Quality Assurance
    • · MS
    • · HPLC
  • Delivery or Next process
    • · complete

List of Modification

List of Modification
N,C-Terminal
Modification		 Special Amino acid Fluorescence/
Dye labeling		 Cyclic
Peptide		 Quenched
Fluorescent Peptide		 Multiple Antigenic
Peptide System		 Protein
Conjugation
Acetylation
(N-Terminal)		 D-Arg, D-Cys, DAsp, D-Asn, D-Glu, D-Gln, D-Ser, D-His, D-Thr, D-Trp, D-Ala, D-Leu, D-Ile, D-Met, D-Pro, D-Val, DPhe, pGlu Biotin (NTerminal,
Y/N
Ahx)		 Disulfide
bridge 1st		 Abz/Tyr (3-NO 2) Asymmetric
4 branches		 KLH
HYNIC
(N-Terminal)		 Hyp, D-Lys,D-Tyr, D-Orn, Orn, Abu, Aib, (D)1-Nal, (D)2- Pal, (D)-4-Cl-Phe, Nva, Nle, Hse, Hcy, Pen, Mpa Biotin (Lys in
sequence)		 Disulfide
bridge 2nd		 EDANS/DABCYL Asymmetric
8 branches		 BSA
DTPA
(N-Terminal)		 N-Methyl amino acid (Ala, Phe, Leu, Ile, Val, Gly, Met) Biotin (without
Lys in
sequence)		 Disulfide
bridge 3rd
Formylation
(N-Terminal)		 Other amino acid Dinitrobenzoylation (Lys) FITC/5-FAM
(N-Terminal,
Y/N Ahx)		 Amide cyclic
(Side chain,
Terminal)
Fatty acid
(N-Terminal)		 Lys(Me 2) Dansyl
(NTerminal, Y/NAhx)
Myristic acid
(N-Terminal)		 Phosphorylation
(Tyr, Ser, Thr, single site)		 MCA (NTerminal)
Palmytolyl
(N-Terminal)		 Tyr(SO3H2) p-Nitroanilide
(Pna, CTerminal)
Benzyloxycarbonylati
on (CBZ)		 Ser(octanoic acid) AMC
(C-Terminal)
Amidation
(C-Terminal)
Succinylation
(Suc, N-Terminal)

Conjugation Service

For small molecules, peptides, and chemicals are used as antigens, there are conjugated to carrier protein to increase immunogenicity.

Carrier protein type : KLH, BSA

Conjugation Linker

  • Sulfo-SMCC: Conjugation using the -SH group (thiol group)
    • - Peptides can be added artificially using cysteine
      • · CysXXXXXXXXXX or XXXXXXXXXXCys (O)
      • · XXXXXCysXXXXX (X)
    • - Coupled with the NH2 group of lysine in the carrier protein

Sulfo-SMCC conjugation
  • Glutaraldehyde: Conjugation using the -NH2 group (free amino group, N-terminal)
    • - Peptides should have no lysine
      • · XXXXXLysXXXXX (X)
    • - Coupled with the NH2 group of lysine in the carrier protein

Glutaraldehyde conjugation
  • The EDC: Conjugation using the -COOH group (carboxyl group, C-terminal)
    • - Peptides should have no aspartic acid and glutamic acid
      • · XXXXXAspXXXXX or XXXXXGluXXXXX (X)
    • - Coupled with the NH2 group of lysine in the carrier protein

EDC conjugation

Small molecules (including peptides) conjugated with a carrier protein are used for ELISA screening in order to confirm antibody production by conjugating carrier protein and other carrier proteins.

  • Ex) Immunization → KLH conjugated peptide
          ELISA screening → BSA conjugated peptide

문의사항

+82-2-2109-1294