AbClon

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Antibody Production Purification Service

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In vitro / In vivo antibody production

AbClon provide mass production of monoclonal antibodies in vitro and in vivo using hybridoma.

Type & volume

Type & volume
Kinds Volume
In vitro 100ml culture ~
In vivo 5 mice ~

Features

  • In vitro benefits
    • - Exclusion of contamination of exogenous antibody, in vitro assay
  • In vivo benefits
    • - Large amount of antibody can be obtained with single production, for development of diagnostic kit
  • Experiment progress data transmission and 1: 1 customized consultation service

Customer Preparation

  • Hybridoma cell
    • - Hybridoma cell stock
    • - Cell and culture information
    • - Production conditions
    • - Antigen (for antibody detection)

Related Service

  • Antibody purification service
    • - IgG purification: Whole IgG purification in culture sup. or ascites using protein A, G
    • - Affinity purification: specific IgG binding to the antigen is purified
  • Epitope mapping
    • - Identification of epitope binding to target using ascites, media or purified antibody
  • Antibody conjugation
    • - Biotin
    • - HRP (Horseradish peroxidase)
    • - FITC (Fluorescein isothiocyanate)
    • - PE (Phycoerythrin)

Service Flow

In vitro production
  • Hybridoma
    Cell thawing & Culture
    • · Cell status check
    • · One week
  • 1st Cell expansion
    • · 1~2 weeks
  • 2nd Cell expansion
    • · 1~2 weeks
  • Culture Supernatant
    Harvast & QC
    • · SDS-PAGE
      confirmation
    • · One week
  • Delivery or
    Next process
    • · complete
In vivo production
  • Mouse Adaptation
    • · Mouse Stabilization
    • · 2 weeks
  • Pristane Injection
    • · One week
  • Hybridoma
    cell Injection
    • · One week
  • Ascites Isolation and QC
    • · SDS-PAGE
      confirmation
    • · 2 weeks
  • Delivery or
    Next process
    • · complete
    • · report

IgG / Affinity antibody purification

Only Hybridoma culture fluid, multiple liquids, and serum will purify the antibody.

Kind and base unit

Kind and base unit
Type Ligand Base unit
IgG purification Protein A, G Ascites or serum: 10 ml
cultured media: 100 ml
Affinity Tablets Protein or peptide

Features

  • Advantages of IgG purification
    • - Efficient purification system provides high purity antibody (over 90% purity)
  • Affinity Tablet Advantage
    • - Only antibodies specifically binding to the antigen are purified
    • - Easy for modification antibody purification

Customer Preparation

  • Serum, ascites, cultured media, etc.
  • Requires Ligand for Affinity Purification (Protein or Peptide)

Related Service

  • Epitope mapping
    • - Identification of epitope binding to target using purified antibody
  • Antibody conjugation
    • - Biotin
    • - HRP (Horseradish peroxidase)
    • - FITC (Fluorescein isothiocyanate)
    • - PE (Phycoerythrin)

Service Flow

IgG purification
  • IgG purification
    • · Protein A & G
    • · One week
  • Concentration & QC
    • · Concentration
    • · Quantitative & SDS-PAGE
    • · One week
  • Delivery or Next process
    • · report
Affinity Tablets
  • Ligand confirmation &
    IgG purification
    • · Protein A & G
    • · One week
  • Affinity purification
    • · Affi-Gel
    • · One week
  • Concentration & QC
    • · Concentration
    • · Quantitative & SDS-PAGE
    • · One week
  • Delivery or Next process
    • · report

문의사항

+82-2-2109-1294