AbClon
Antibody Services
Monoclonal Antibody
Monoclonal antibody is an antibody derived from one B cell. Since it recognizes only one epitope,
it is widely used not only for research but also for diagnosis and treatment. Peptides or purified
proteins are mainly used as antigens. Antibodies can also be produced by various substances such
as chemical or cell. Compared with Polyclonal Antibody, the antibody development period is long
and the process is complicated. However, the developed antibody has the advantage of
continuous production with the same quality. The Monoclonal Antibody Next Innovation (MANI)
protocol, developed independently by AbClon, is a dramatic increase in the number of positive
clones that respond to antigens compared to SOP.
We also offer in vivo ascites / purification or in vitro culture / purification services for the mass
production of monoclonal antibodies with activity identified. In order to increase the success rate
of antibody production, the form of antigen should be close to that of native protein, and its size
should be more than 20 kDa. Therefore, the success rate of antibody production is higher in order of native protein> recombinant protein> peptide. If the size of the protein is small, you can increase the size by attaching the tag and immunize and select the antigen-specific antibody by negative selection for the tag protein.
it is widely used not only for research but also for diagnosis and treatment. Peptides or purified
proteins are mainly used as antigens. Antibodies can also be produced by various substances such
as chemical or cell. Compared with Polyclonal Antibody, the antibody development period is long
and the process is complicated. However, the developed antibody has the advantage of
continuous production with the same quality. The Monoclonal Antibody Next Innovation (MANI)
protocol, developed independently by AbClon, is a dramatic increase in the number of positive
clones that respond to antigens compared to SOP.
We also offer in vivo ascites / purification or in vitro culture / purification services for the mass
production of monoclonal antibodies with activity identified. In order to increase the success rate
of antibody production, the form of antigen should be close to that of native protein, and its size
should be more than 20 kDa. Therefore, the success rate of antibody production is higher in order of native protein> recombinant protein> peptide. If the size of the protein is small, you can increase the size by attaching the tag and immunize and select the antigen-specific antibody by negative selection for the tag protein.
Service Details
| Subject | Animal | Guaranteed clone | AbClon offerings |
|---|---|---|---|
| SOP | 4 mice | 2 clones out of 5 clones | Final serum (~500 ul / mouse) Final cell culture sup. (~10 ml / clone) Final stock (5 vials / clone) |
| MANI Protocol | 5 clones out of 10 clones |
※ ELISA guarantee for antigen
Features
- MANI protocol can acquire more clones than SOP
- MANI protocol has shorter development period than SOP
- After the fusion screening, culture sup can be provided (WB, IP, FACS, etc.)
- Cell cloning can be performed by selecting desired clones
- Experiment progress step by step data transmission and 1: 1 customized consultation service
- Continuous antibody production with the same quality
- High specificity with antibodies reactive to one epitope
Customer Preparation
- Antigen protein retention
- - Required amount: 2.5-3 mg
- - Concentration: 1.0mg / ml or more
- - Purity: 95% or more (based on SDS-PAGE)
- - Recommended size: 20kDa or more
- peptide sequence or protein sequence retention
- - Possible to link gene synthesis service with protein expression purification service
- · Sequencing and prediction services available
- · Recommended length: 10 to 20 mer
- · Required amount: 5mg
- - Possible to link gene synthesis service with protein expression purification service
Related Service
- Antibody production purification Service
- - In vitro production: Antibody production through hybridoma culture
- - In vivo production: Antibody production through ascites in mouse abdominal cavity
- - IgG purification: Protein A, G or whole IgG purification in ascites or culture sup.
- - Affinity purification: only specific IgG binding to the antigen is purified
- Epitope mapping
- - Identify epitope using antibody and culture sup.
- Antibody conjugation
- - Biotin
- - HRP (horseradish peroxidase)
- - FITC (fluorescein isothiocyanate)
- - PE (phycoerythrin)
Service Flow
Polyclonal Antibody
Polyclonal antibody is an antibody that recognizes various epitopes. It can be produced at low
cost in a short period of time and widely used in various research fields such as WB, IHC, ICC,
and ELISA. Peptides and purified proteins are mainly used as antigens. Antibodies can also be
produced by various substances such as chemical or cell. Antibodies are provided in the form
of a serum and can be purified to improve quality after checking antibody activity.
cost in a short period of time and widely used in various research fields such as WB, IHC, ICC,
and ELISA. Peptides and purified proteins are mainly used as antigens. Antibodies can also be
produced by various substances such as chemical or cell. Antibodies are provided in the form
of a serum and can be purified to improve quality after checking antibody activity.
Host type and Serum quantity
| Host | Pre-immune serum | Test serum | Final serum |
|---|---|---|---|
| 2 Rabbit | ~ 500 ul | ~ 500 ul | ~ 40 ml |
| 4 mice | ~ 500 ul |
※ The amount of serum given per animal may vary depending on the animal's condition.
Features
- Test serum can be provided to check immune stage antibody activity
- Experiment progress step by step data transmission and 1: 1 customized consultation service
- Low background of pre-immune serum using high quality animal
- Antibodies that recognize multiple epitopes can be used for various experiments
- Can be manufactured in a short time at low cost
Customer Preparation
- Antigen protein retention
- - Required amount : 2.5-3 mg
- - Concentration : 1.0mg / ml or more
- - Purity : 95% or more (based on SDS-PAGE)
- - Recommended size : 20kDa or more
- Retention of peptide sequence or protein sequence
- - Possible to link gene synthesis service with protein
- Expression purification service
- - Peptide synthesis and conjugation services available
- · Sequencing and prediction services available
- · Recommended length: 10 to 20 mer
- · Required amount: 5mg
AbClon offerings
- Pre-immune serum
- Test serum
- Final serum
- ELISA data
Link Service
- Antibody purification service
- - IgG Purification : Whole IgG purification in serum using Protein A, G
- - Affinity purification : only specific IgG binding to the antigen is purified
- Epitope mapping
- - Identification of epitope binding to target using serum or purified antibody
- Antibody conjugation
- - Biotin
- - HRP (Horseradish peroxidase)
- - FITC (Fluorescein isothiocyanate)
- - PE (Phycoerythrin)
Service Flow
Antibody library
The Rz library is a human synthetic library developed by AbClon and has two types, Fab and scFv. Antibodies in the form of Fabs or scFvs that bind to a wide variety of antigens can be provided and can be screened for antibodies within a short time (6 weeks).
Trinucleotide mutagenesis library has been used to prevent the generation of stop codon to maximize the success rate of antibody development. Developing Antibodies Using Hybridoma Antibodies can be developed in a short period of time with fewer antigens.
Library type
Features
- Human antibody library
- Stable antibody scaffold
- High functionality (trinucleotide mutagenesis)
- High diversity of CDRs (> 1*1010)
- Outstanding success in drug discovery
Customer Preparation
- Antigen protein retention
- - Required amount: 100ug
- - Purity: 95% or more (based on SDS-PAGE)
- Antigen protein sequence
- - Possible to link gene synthesis service with protein expression purification service
AbClon offerings
- Provide Fab or scFv (100 ug) (max 5 (based on antigen ELISA))
- ELISA Test REPORT
- Variable region sequence information
- Variable region Fragment
Library Case
- Antibodies in-house developed through Rz Library
| Disease Field | Target (Target size) |
|---|---|
| Oncology | A (180 kDa) |
| B (175 kDa) | |
| C (200 kDa) | |
| D (60 kDa) | |
| Autoimmune | E (80 kDa) |
| F (17 kDa) | |
| G (22 kDa) | |
| H (50 kDa) |
Link Service
- Antibody Engineering
- - Full IgG conversion: Produced in complete IgG form
- Epitope mapping
- - Epitope confirmation using Fab or scFv
- Efficacy test
- - In vitro
- - In vivo
Service Flow
+82-2-2109-1294
kdlee@abclon.com
하단영역
AbClon Inc. #1401 ,285, Digital-ro, Guro-gu, Seoul , Korea TEL : +82-2-2109-1269 FAX : +82-2-2109-1296
Copyright © 2017. AbClon Inc. All right reserved.
Copyright © 2017. AbClon Inc. All right reserved.