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Affinity measurement

The Octet analysis system is a fast and accurate measure of antibody binding affinity (KD). Eight samples can be analyzed at the same time, easy sorting of antibodies by K-off ranking, and accurate measurement of antibody affinity change during humanization and affinity maturation.

Octet analysis can measure binding affinity between antibody and antigen as well as binding affinity between ligand and receptor. It is also used for antibody characterization such as epitope binning.

Octet analysis is based on BioLayer Interferometry (BLI) technology, which uses the difference in length of the white light wavelengths reflected from the internal reference layer and the two surfaces of the immobilized protein layer.


  • Short running time
  • Measurable in lysate form
  • Multiple concentrations can be measured simultaneously
  • Reusable sample
  • Ability to monitor data in real time

Customer Preparation

  • Antibody
    • - Required amount: 100ug
    • - Purity: more than 90%
    • - Concentration: 1 mg / ml
  • Antigen
    • - Amount needed: 100 ug
    • - Monomeric protein
    • - Purity: more than 90%
    • - Size: over 5kDa
    • - Concentration: 1 mg/ml
    • - Antibody and antigen response

AbClon provided

  • data


  • Kinetic characterization
  • Protein-Protein interaction
  • Antibody binder screening
  • Epitope binning
  • Affinity maturation
  • Assay development

Flow & Graph

Antibody Conjugation

AbClon is conjugating the antibody so that it can be used in various fields.


  • HRP Conjugation
  • FITC Conjugation
  • Biotin Conjugation
  • PE Conjugation
  • Antibody-Resin Conjugation